Alternative Egg Protein
P. pastoris Expression System
The client approached us without a scalable manufacturing process, as development had been limited to shake flask experiments conducted in an academic laboratory and had not yet been transferred to fermenters.
To ensure industrial feasibility, a structured transition from laboratory experimentation to controlled fermentation was required.
The project centered on Pichia pastoris fermentation scale-up, converting an early-stage academic process into a scalable and industrially viable production platform.
Process Development & Optimization
With close technical support from our team, the client successfully established a basic fermentation process in a laboratory-scale fermenter. This marked a critical milestone in the Pichia pastoris fermentation scale-up journey, converting an academic proof-of-concept into a structured and scalable bioproduction platform.
Following the initial technology transfer, we continued supporting the client in optimizing fermentation parameters at lab scale to further advance the Pichia pastoris fermentation scale-up program. During this phase, a refined feeding regime was developed, resulting in a significant reduction in glycerol consumption.
This optimization delivered two key advantages: reduced media costs and mitigation of potential regulatory concerns associated with the use of large quantities of glycerol. By addressing both economic and regulatory aspects, the process was strategically positioned for sustainable industrial scale-up.
Scale-Up
The optimized process was successfully scaled to an 80L fermenter, demonstrating robustness beyond laboratory conditions and validating readiness for continued industrial progression.
Project Duration: 9 months
